Title: Developing an Imaging Bi-Spectrometer for Fluorescent Materials
نویسنده
چکیده
Fluorescent effects have been observed for thousands of years. Stokes, in 1852, began the science of fluorescence culminating in his law of fluorescence, which explained that fluorescence emission occurs at longer wavelengths than the excitation wavelength. This phenomenon is observed extensively in the art world. Daylight fluorescent colors known as Day-Glo have become an artistic medium since the 1960s. Modern artists exploit these saturated and brilliant colors to glitter their painting. Multispectral imaging as a noninvasive technique has been used for archiving by museums and cultural-heritage institutions for about a decade. The complex fluorescence phenomenon has been often ignored in the multispectral projects. The ignored fluorescence results in errors in digital imaging of artwork containing fluorescent colors. The illuminantdependency of the fluorescence radiance makes the fluorescence colorimetry and consequently spectral imaging more complex. In this dissertation an abridged imaging bi-spectrometer for artwork containing both fluorescent and non-fluorescent colors was developed. The method developed included two stages of reconstruction of the spectral reflected radiance factor and prediction of the fluorescent radiance factor. The estimation of the reflected radiance factor as a light source independent component was achieved by imaging with a series of short-wavelength cutoff filters placed in the illumination path. The fluorescent radiance factor, a light source dependent component, was estimated based on a proposed model, the abridged two-monochromator method. The abridged two-monochromator method was developed for reconstructing the bi-spectral matrix of a fluorescent color based on a calibrated UV-fluorescence imaging. In this way, one could predict the fluorescence radiance factor under any desired illuminant and consequently a better color evaluation and rendering could be obtained. Furthermore, this method easily fitted in a general system for spectral imaging of paintings containing both fluorescent and non-fluorescent colors. The abridged two-monochromator method could predict fluorescent radiance factor of a fluorescent color via prediction of the true emission and the number of absorbed quanta by a fluorescing specimen for a given viewing light source. The superiority of the abridged fluorescence spectral imaging to the traditional spectral and colorimetric imaging for a few light sources was confirmed using fluorescent and non-fluorescent targets. Additionally, an exploratory visual experiment using a paired-comparison method was performed to evaluate the performance of the abridged fluorescence spectral imaging in comparison to the traditional spectral and colorimetric imaging for rendering images of a reference painting. The abridged fluorescence spectral imaging had better performance than traditional spectral and colorimetric imaging in rendering images for daylight.
منابع مشابه
Graphene Quantum Dots-Coated Bismuth Nanoparticles for X-ray CT Imaging-Guided Photothermal therapy of Cancer Cells
Introduction: Theranostic nanoparticles, which integrate both diagnostic and therapeutic capabilities into one nanoagent, has great promise to ablate more effective tumoral tissue by optimizing and real-time monitoring treatment interventions, as well as monitoring therapeutic response to corresponding effect. Multifunctional theranostic nanoagent based on graphene quantum dots...
متن کاملStatic Hyperspectral Fluorescence Imaging of Viscous Materials Based on a Linear Variable Filter Spectrometer
This paper presents a low-cost hyperspectral measurement setup in a new application based on fluorescence detection in the visible (Vis) wavelength range. The aim of the setup is to take hyperspectral fluorescence images of viscous materials. Based on these images, fluorescent and non-fluorescent impurities in the viscous materials can be detected. For the illumination of the measurement object...
متن کاملMultifunctional GQDs-Coated Fe/Bi Nanohybrids for CT/MR Dual Imaging and in vitro Photothermal Therapy
Introduction: The multipurpose nanocomposites have gained growing biomedical attention as promising nanotheranostics to improve The effectiveness of cancer treatment, which concurrently combine advantages of the therapeutic and diagnostic techniques into one nanosystem. The “all-in on” probes not only help to ablate cancerous tumors, but also allow to optimize and monitoring of...
متن کاملReal-time hyperspectral fluorescence imaging of pancreatic β-cell dynamics with the image mapping spectrometer.
The development of multi-colored fluorescent proteins, nanocrystals and organic fluorophores, along with the resulting engineered biosensors, has revolutionized the study of protein localization and dynamics in living cells. Hyperspectral imaging has proven to be a useful approach for such studies, but this technique is often limited by low signal and insufficient temporal resolution. Here, we ...
متن کاملMultimodal, multiplex, Raman spectroscopy of alcohol in diffuse, fluorescent media
Optical diagnostics in biological materials are hindered by fluorescence and scattering. We have developed a multimodal, multiplex, coded-aperture Raman spectrometer to detect alcohol in a lipid tissue phantom solution.
متن کامل